Metal chelating properties of adenylate kinase from Paracoccus denitrificans
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چکیده
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Purification and properties of methylamine dehydrogenase from Paracoccus denitrificans.
Methylamine dehydrogenase from Paracoccus denitrificans was purified to homogeneity in two steps from the periplasmic fraction of methylamine-grown cells. The enzyme exhibited a pI value of 4.3 and was composed of two 46,700-dalton subunits and two 15,500-dalton subunits. Each small subunit possessed a covalently bound pyrrolo-quinoline quinone prosthetic group. The amino acid compositions of t...
متن کاملTurnover of cytochrome c oxidase from Paracoccus denitrificans.
The heme aa3 type cytochrome oxidase from Paracoccus denitrificans incorporated into vesicles with phospholipid reacts during turnover much as the oxidase from mitochondria does. The spectrophotometric changes observed at various wavelengths are closely similar, and the rate is about one-half of that for beef heart oxidase under the same conditions. The rate of appearance of oxidized cytochrome...
متن کاملThe cytochrome c oxidase from Paracoccus denitrificans does not change the metal center ligation upon reduction.
Cytochrome c oxidase catalyzes the reduction of oxygen to water. This process is accompanied by the vectorial transport of protons across the mitochondrial or bacterial membrane ("proton pumping"). The mechanism of proton pumping is still a matter of debate. Many proposed mechanisms require structural changes during the reaction cycle of cytochrome c oxidase. Therefore, the structure of the cyt...
متن کاملKinetics of iron acquisition from ferric siderophores by Paracoccus denitrificans.
The kinetics of iron accumulation by iron-starved Paracoccus denitrificans during the first 2 min of exposure to 55Fe-labeled ferric siderophore chelates is described. Iron is acquired from the ferric chelate of the natural siderophore L-parabactin in a process exhibiting biphastic kinetics by Lineweaver-Burk analysis. The kinetic data for 1 microM less than [Fe L-parabactin] less than 10 micro...
متن کاملDetermination of the Paracoccus denitrificans SOS box.
By gel retardation experiments with crude cell extracts of Paracoccus denitrificans it was demonstrated that a protein specifically binds to the promoter of the P. denitrificans recA gene. PCR mutagenesis of the recA promoter showed that the GAACN7GAAC motif is required for the formation of the DNA-protein complex. This protein also binds to the GTTCN7GTTC motif, which is present in the promote...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 1998
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/11.10.917